Products

Calpain Activity Assay & Inhibitor Screening Kits

Activation of calpain is involved in many physiological and pathological processes (e.g., apoptosis).

The Calpain Activity Assay Kit provides a simple and convenient means for analyzing calpain activity in apoptotic and other samples. The assay is based on fluorometric detection and quantitation of the cleavage of a calpain substrate at 505 nm using a fluorometer or a fluorescence plate reader. The kit contains both positive and negative controls. Comparison of the fluorescence intensity from a treated sample with a normal control allows determination of the changes in calpain activity.

 

The fluorometric Calpain-1 Inhibitor Screening Kit includes active Calpain-1 enzyme and a synthetic    Calpain-1 substrate. It provides a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of Calpain-1 inhibitors. In the presence of potent Calpain-1 inhibitors, the hydrolyzation of the calpain substrate will be impeded. A control is included to compare the efficacy of test inhibitors.

 

Active calpain enzyme as well as calpain inhibitors and antibodies are also available separately.

References, Questions & Answers

Product Name Description Size Catalog Number Information Prices
Calpain Activity Assay Kit Kit provides a simple and convenient means for analyzing calpain activity in apoptotic and other samples 100 assays PK-CA577-K240 click to select country
Calpain 1 Inhibitor Screening Kit Allows fast screening of potential human calpain 1 inhibitors 100 assays PK-CA577-K244 click to select country
Calpain-1, human, (native) Active Human Calpain 1 100 µg PK-RP577-1134 click to select country
Calpain Inhibitor Z-LLY-FMK (10 mM) Provided in the ready-to-use form 20 µl PK-CA577-1125-20C click to select country
Calpain Inhibitor I A cell-permeable inhibitor of calpain I, calpain II, cathepsin B, and cathepsin L. 5 mg PK-CA577-1834-5 click to select country
Calpain Inhibitor I A cell-permeable inhibitor of calpain I, calpain II, cathepsin B, and cathepsin L. 25 mg PK-CA577-1834-25 click to select country
Calpain Inhibitor II A cell-permeable inhibitor of calpain 1 , calpain 2 which also inhibits cathepsin B, and cathepsin L. 5 mg PK-CA577-1835-5 click to select country
Calpain Inhibitor II A cell-permeable inhibitor of calpain 1 , calpain 2 which also inhibits cathepsin B, and cathepsin L. 25 mg PK-CA577-1835-25 click to select country