The PromoKine Caspase Screening Kit (FC) provides a convenient means for detecting the activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D2R), a reported substrate for members of caspase family proteases. The caspase substrate D2R is nonfluorescent. However, upon cleavage of the substrate by cellular activated caspases, the released rhodamine 110 gives rise to fluorescence that can be measured by flow cytometry at excitation of 488 nm and emission of 530 nm.
