Applications

Apoptosis screening
Quantification of caspase activity

Features

Highly sensitive and specific
Fast and convenient
Compatible with standard lab equipment
Suited for automation

Caspase Fluorometric and Colorimetric Assay Kits

The Caspase Fluorometric and Colorimetric Assay Kits are intented for convenient and sensitive quantification of caspase activity in cell lysates. The kits utilize synthetic peptides which mimic the optimal substrate for distinct caspases and are labeled with either 7-amino-4-trifluoromethyl coumarin (AFC, fluorometric assay) or p-nitroanilide (pNA, colorimetric assay). Activated caspases in apoptotic cells cleave specifically their synthetic substrates resulting in release of free AFC or pNA, which is then quantified using a fluorometer or spectrophotometer, respectively. The kits are fast, simple, straightforward, and can be adapted for 96-well plates.

In addition, PromoKine offers Caspase Fluorometric & Colorimetric Assay Kits for quantifying caspase-8 activity in mammalian cells by either fluorescence or absorbance in a fast and simple procedure. The kits utilize the caspase substrate (Ac-IETD)₂-R110 that is fluorogenic as well as chromogenic.

The Caspase-8 Fluorometric Assay Kits (HTS) have been designed for economic high-throughput applications. They use the fluorogenic and chromogenic substrate (Ac-IETD)₂-R110 and include specific caspase inhibitors as negative controls.

Knowledge Base

To view questions and answers for this product, please click here.

Product Name	Description	Size	Catalog Number
2X Reaction Buffer	Optimized buffer for caspase activity assay reactions	80 ml	PK-CA577-1068-80
2X Reaction Buffer	Optimized buffer for caspase activity assay reactions	20 ml	PK-CA577-1068-20
Caspase-1 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	100 assays	PK-CA577-K111-100
Caspase-1 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	200 assays	PK-CA577-K111-200
Caspase-1 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	25 assays	PK-CA577-K111-25
Caspase-1 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	400 assays	PK-CA577-K111-400
Caspase-1 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	100 assays	PK-CA577-K110-100
Caspase-1 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	200 assays	PK-CA577-K110-200
Caspase-1 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	25 assays	PK-CA577-K110-25
Caspase-1 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	400 assays	PK-CA577-K110-400
Caspase-10 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	100 assays	PK-CA577-K125-100
Caspase-10 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	25 assays	PK-CA577-K125-25
Caspase-10 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	100 assays	PK-CA577-K124-100
Caspase-10 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	25 assays	PK-CA577-K124-25
Caspase-12 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	100 assays	PK-CA577-K139-100
Caspase-12 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	25 assays	PK-CA577-K139-25
Caspase-2 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	100 assays	PK-CA577-K117-100
Caspase-2 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	200 assays	PK-CA577-K117-200
Caspase-2 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	25 assays	PK-CA577-K117-25
Caspase-2 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	400 assays	PK-CA577-K117-400
Caspase-2 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	100 assays	PK-CA577-K116-100
Caspase-2 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	200 assays	PK-CA577-K116-200
Caspase-2 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	25 assays	PK-CA577-K116-25
Caspase-2 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	400 assays	PK-CA577-K116-400
Caspase-3 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	100 assays	PK-CA577-K106-100
Caspase-3 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	200 assays	PK-CA577-K106-200
Caspase-3 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	25 assays	PK-CA577-K106-25
Caspase-3 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	400 assays	PK-CA577-K106-400
Caspase-3 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	100 assays	PK-CA577-K105-100
Caspase-3 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	200 assays	PK-CA577-K105-200
Caspase-3 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	25 assays	PK-CA577-K105-25
Caspase-3 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	400 assays	PK-CA577-K105-400
Caspase-4 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	100 assays	PK-CA577-K127-100
Caspase-4 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	200 assays	PK-CA577-K127-200
Caspase-4 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	25 assays	PK-CA577-K127-25
Caspase-4 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	100 assays	PK-CA577-K126-100
Caspase-4 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	200 assays	PK-CA577-K126-200
Caspase-4 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	25 assays	PK-CA577-K126-25
Caspase-5 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	100 assays	PK-CA577-K123-100
Caspase-5 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	200 assays	PK-CA577-K123-200
Caspase-5 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	25 assays	PK-CA577-K123-25
Caspase-5 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	400 assays	PK-CA577-K123-400
Caspase-5 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	100 assays	PK-CA577-K122-100
Caspase-5 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	200 assays	PK-CA577-K122-200
Caspase-5 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	25 assays	PK-CA577-K122-25
Caspase-5 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	400 assays	PK-CA577-K122-400
Caspase-6 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	100 assays	PK-CA577-K115-100
Caspase-6 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	200 assays	PK-CA577-K115-200
Caspase-6 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	25 assays	PK-CA577-K115-25
Caspase-6 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	400 assays	PK-CA577-K115-400
Caspase-6 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	100 assays	PK-CA577-K114-100
Caspase-6 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	200 assays	PK-CA577-K114-200
Caspase-6 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	25 assays	PK-CA577-K114-25
Caspase-6 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	400 assays	PK-CA577-K114-400
Caspase-8 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	100 assays	PK-CA577-K113-100
Caspase-8 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	200 assays	PK-CA577-K113-200
Caspase-8 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	25 assays	PK-CA577-K113-25
Caspase-8 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	400 assays	PK-CA577-K113-400
Caspase-8 Fluorometric & Colorimetric Assay Kit	Kit for measuring caspase activity by spectrometer, fluorometer or plate reader	25 assays	PK-CA707-30011-1
Caspase-8 Fluorometric & Colorimetric Assay Kit	Kit for measuring caspase activity by spectrometer, fluorometer or plate reader	100 assays	PK-CA707-30011-2
Caspase-8 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	100 assays	PK-CA577-K112-100
Caspase-8 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	200 assays	PK-CA577-K112-200
Caspase-8 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	25 assays	PK-CA577-K112-25
Caspase-8 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	400 assays	PK-CA577-K112-400
Caspase-8 Fluorometric Assay Kit (HTS)	Kit for measuring caspase activity by fluorometer or plate reader	1 ml	PK-CA707-30012-1
Caspase-8 Fluorometric Assay Kit (HTS)	Kit for measuring caspase activity by fluorometer or plate reader	10 ml	PK-CA707-30012-2
Caspase-8 Fluorometric Assay Kit (HTS)	Kit for measuring caspase activity by fluorometer or plate reader	100 ml	PK-CA707-30012-3
Caspase-9 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	100 assays	PK-CA577-K119-100
Caspase-9 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	200 assays	PK-CA577-K119-200
Caspase-9 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	25 assays	PK-CA577-K119-25
Caspase-9 Colorimetric Assay Kit	Kit for measuring caspase activity by spectrophotometer or plate reader	400 assays	PK-CA577-K119-400
Caspase-9 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	100 assays	PK-CA577-K118-100
Caspase-9 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	200 assays	PK-CA577-K118-200
Caspase-9 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	25 assays	PK-CA577-K118-25
Caspase-9 Fluorometric Assay Kit	Kit for measuring caspase activity by fluorometer or plate reader	400 assays	PK-CA577-K118-400
Cell Lysis Buffer	For lysis of tissue and cells, e.g. in caspase activity assays	100 ml	PK-CA577-1067-100
Cell Lysis Buffer	For lysis of tissue and cells, e.g. in caspase activity assays	400 ml	PK-CA577-1067-400
Dilution Buffer	For use with colorimetric caspase activity assays	100 ml	PK-CA577-1066-100
Dilution Buffer	For use with colorimetric caspase activity assays	400 ml	PK-CA577-1066-500
DTT (1 M)	Dithiothreitol Solution (1 M)	1 ml	PK-CA577-1201-1