Products

Caspase Fluorometric and Colorimetric Assay Kits

The Caspase Fluorometric and Colorimetric Assay Kits are intented for convenient and sensitive quantification of caspase activity in cell lysates. The kits utilize synthetic peptides which mimic the optimal substrate for distinct caspases and are labeled with either 7-amino-4-trifluoromethyl coumarin (AFC, fluorometric assay) or p-nitroanilide (pNA, colorimetric assay). Activated caspases in apoptotic cells cleave specifically their synthetic substrates resulting in release of free AFC or pNA, which is then quantified using a fluorometer or spectrophotometer, respectively. The kits are fast, simple, straightforward, and can be adapted for 96-well plates.

 


 

In addition, PromoKine offers Caspase Fluorometric & Colorimetric Assay Kits for quantifying caspase-3 or caspase-8 activity in mammalian cells by either fluorescence or absorbance in a fast and simple procedure. The kits utilize the caspase substrates (Ac-DEVD)2-R110 or (Ac-IETD)2-R110 which are fluorogenic as well as chromogenic*.

The Caspase-3 and Caspase-8 Fluorometric Assay Kits (HTS) have been designed for economic high-throughput applications. They also use the fluorogenic and chromogenic substrates (Ac-DEVD)2-R110 or (Ac-IETD)2-R110 and include specific caspase inhibitors as negative controls. They are ideally for high-throughput microtitre plate assays using either an absorbance or a fluorescence plate reader*.

*Note: Although fluorometric detection of the end products is preferred due to the superior sensitivity, detection by absorbance is also feasible as well. In fact, the extinction coefficient of R110 is 10 times higher than that of p-nitroaniline (pNA), a dye commonly used in chromogenic substrates. Therefore, R110-based substrates are significantly more sensitive than pNA-based substrates, even by colorimetric detection. The intensity of the fluorescent or colorimetric signal generated from the assay is proportional to the caspase-3 activity present in the sample.

References, Questions & Answers

Product Name Description Size Catalog Number Information Prices
2X Reaction Buffer Optimized buffer for caspase activity assay reactions 80 ml PK-CA577-1068-80 click to select country
2X Reaction Buffer Optimized buffer for caspase activity assay reactions 20 ml PK-CA577-1068-20 click to select country
Caspase-1 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 100 assays PK-CA577-K111-100 click to select country
Caspase-1 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 200 assays PK-CA577-K111-200 click to select country
Caspase-1 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 25 assays PK-CA577-K111-25 click to select country
Caspase-1 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 400 assays PK-CA577-K111-400 click to select country
Caspase-1 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 100 assays PK-CA577-K110-100 click to select country
Caspase-1 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 200 assays PK-CA577-K110-200 click to select country
Caspase-1 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 25 assays PK-CA577-K110-25 click to select country
Caspase-1 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 400 assays PK-CA577-K110-400 click to select country
Caspase-10 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 100 assays PK-CA577-K125-100 click to select country
Caspase-10 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 200 assays PK-CA577-K125-200 click to select country
Caspase-10 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 25 assays PK-CA577-K125-25 click to select country
Caspase-10 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 400 assays PK-CA577-K125-400 click to select country
Caspase-10 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 100 assays PK-CA577-K124-100 click to select country
Caspase-10 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 200 assays PK-CA577-K124-200 click to select country
Caspase-10 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 25 assays PK-CA577-K124-25 click to select country
Caspase-10 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 400 assays PK-CA577-K124-400 click to select country
Caspase-12 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 100 assays PK-CA577-K139-100 click to select country
Caspase-12 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 25 assays PK-CA577-K139-25 click to select country
Caspase-2 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 100 assays PK-CA577-K117-100 click to select country
Caspase-2 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 200 assays PK-CA577-K117-200 click to select country
Caspase-2 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 25 assays PK-CA577-K117-25 click to select country
Caspase-2 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 400 assays PK-CA577-K117-400 click to select country
Caspase-2 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 100 assays PK-CA577-K116-100 click to select country
Caspase-2 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 200 assays PK-CA577-K116-200 click to select country
Caspase-2 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 25 assays PK-CA577-K116-25 click to select country
Caspase-2 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 400 assays PK-CA577-K116-400 click to select country
Caspase-3 Fluorometric & Colorimetric Assay Kit Kit for measuring caspase-3 activity by spectrometer, fluorometer or plate reader 100 assays PK-CA707-30008-2 click to select country
Caspase-3 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 100 assays PK-CA577-K106-100 click to select country
Caspase-3 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 200 assays PK-CA577-K106-200 click to select country
Caspase-3 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 25 assays PK-CA577-K106-25 click to select country
Caspase-3 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 400 assays PK-CA577-K106-400 click to select country
Caspase-3 Fluorometric & Colorimetric Assay Kit Kit for measuring caspase-3 activity by spectrometer, fluorometer or plate reader 25 assays PK-CA707-30008-1 click to select country
Caspase-3 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 100 assays PK-CA577-K105-100 click to select country
Caspase-3 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 200 assays PK-CA577-K105-200 click to select country
Caspase-3 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 25 assays PK-CA577-K105-25 click to select country
Caspase-3 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 400 assays PK-CA577-K105-400 click to select country
Caspase-3 Fluorometric Assay Kit (HTS) Kit for measuring caspase-3 activity by fluorometer, photometer or plate reader 1 ml PK-CA707-30009-1 click to select country
Caspase-3 Fluorometric Assay Kit (HTS) Kit for measuring caspase-3 activity by fluorometer, photometer or plate reader 10 ml PK-CA707-30009-2 click to select country
Caspase-3 Fluorometric Assay Kit (HTS) Kit for measuring caspase-3 activity by fluorometer, photometer or plate reader 100 ml PK-CA707-30009-3 click to select country
Caspase-4 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 100 assays PK-CA577-K127-100 click to select country
Caspase-4 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 200 assays PK-CA577-K127-200 click to select country
Caspase-4 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 25 assays PK-CA577-K127-25 click to select country
Caspase-4 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 400 Assays PK-CA577-K127-400 click to select country
Caspase-4 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 100 assays PK-CA577-K126-100 click to select country
Caspase-4 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 200 assays PK-CA577-K126-200 click to select country
Caspase-4 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 25 assays PK-CA577-K126-25 click to select country
Caspase-4 Fluorometric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 400 Assays PK-CA577-K126-400 click to select country
Caspase-5 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 100 assays PK-CA577-K123-100 click to select country
Caspase-5 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 200 assays PK-CA577-K123-200 click to select country
Caspase-5 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 25 assays PK-CA577-K123-25 click to select country
Caspase-5 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 400 assays PK-CA577-K123-400 click to select country
Caspase-5 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 100 assays PK-CA577-K122-100 click to select country
Caspase-5 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 200 assays PK-CA577-K122-200 click to select country
Caspase-5 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 25 assays PK-CA577-K122-25 click to select country
Caspase-5 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 400 assays PK-CA577-K122-400 click to select country
Caspase-6 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 100 assays PK-CA577-K115-100 click to select country
Caspase-6 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 200 assays PK-CA577-K115-200 click to select country
Caspase-6 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 25 assays PK-CA577-K115-25 click to select country
Caspase-6 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 400 assays PK-CA577-K115-400 click to select country
Caspase-6 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 100 assays PK-CA577-K114-100 click to select country
Caspase-6 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 200 assays PK-CA577-K114-200 click to select country
Caspase-6 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 25 assays PK-CA577-K114-25 click to select country
Caspase-6 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 400 assays PK-CA577-K114-400 click to select country
Caspase-8 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 100 assays PK-CA577-K113-100 click to select country
Caspase-8 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 200 assays PK-CA577-K113-200 click to select country
Caspase-8 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 25 assays PK-CA577-K113-25 click to select country
Caspase-8 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 400 assays PK-CA577-K113-400 click to select country
Caspase-8 Fluorometric & Colorimetric Assay Kit Kit for measuring caspase-8 activity by fluorometer, photometer or plate reader 25 assays PK-CA707-30011-1 click to select country
Caspase-8 Fluorometric & Colorimetric Assay Kit Kit for measuring caspase-8 activity by fluorometer, photometer or plate reader 100 assays PK-CA707-30011-2 click to select country
Caspase-8 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 100 assays PK-CA577-K112-100 click to select country
Caspase-8 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 200 assays PK-CA577-K112-200 click to select country
Caspase-8 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 25 assays PK-CA577-K112-25 click to select country
Caspase-8 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 400 assays PK-CA577-K112-400 click to select country
Caspase-8 Fluorometric Assay Kit (HTS) Kit for measuring caspase-8 activity by fluorometer, photometer or plate reader 1 ml PK-CA707-30012-1 click to select country
Caspase-8 Fluorometric Assay Kit (HTS) Kit for measuring caspase-8 activity by fluorometer, photometer or plate reader 10 ml PK-CA707-30012-2 click to select country
Caspase-8 Fluorometric Assay Kit (HTS) Kit for measuring caspase-8 activity by fluorometer, photometer or plate reader 100 ml PK-CA707-30012-3 click to select country
Caspase-9 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 100 assays PK-CA577-K119-100 click to select country
Caspase-9 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 200 assays PK-CA577-K119-200 click to select country
Caspase-9 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 25 assays PK-CA577-K119-25 click to select country
Caspase-9 Colorimetric Assay Kit Kit for measuring caspase activity by spectrophotometer or plate reader 400 assays PK-CA577-K119-400 click to select country
Caspase-9 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 100 assays PK-CA577-K118-100 click to select country
Caspase-9 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 200 assays PK-CA577-K118-200 click to select country
Caspase-9 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 25 assays PK-CA577-K118-25 click to select country
Caspase-9 Fluorometric Assay Kit Kit for measuring caspase activity by fluorometer or plate reader 400 assays PK-CA577-K118-400 click to select country
Cell Lysis Buffer For lysis of tissue and cells, e.g. in caspase activity assays 100 ml PK-CA577-1067-100 click to select country
Cell Lysis Buffer For lysis of tissue and cells, e.g. in caspase activity assays 400 ml PK-CA577-1067-400 click to select country
Dilution Buffer For use with colorimetric caspase activity assays 100 ml PK-CA577-1066-100 click to select country
Dilution Buffer For use with colorimetric caspase activity assays 400 ml PK-CA577-1066-500 click to select country
DTT (1 M) Dithiothreitol Solution (1 M) 1 ml PK-CA577-1201-1 click to select country