Caspase Drug Screening Kits

Inhibition of caspases has been shown to delay apoptosis, implicating a potential role in drug screening efforts. PromoKine provides a full line of Caspase Drug Screening Kits for effective screening of caspase inhibitors using fluorometric methods. The assays utilize peptide substrates containing consensus cleavage sequences specific for each caspase, labeled with AFC (7-amino-4-trifluoromethyl coumarin). Active caspases (provided in the kits) cleave the synthetic substrates to release free AFC which can then be quantified using a microtiter plate reader. Compounds to be screened can directly be added to the reaction and the level of caspase inhibition can then be determined. The assays are simple, straightforward, and can be performed directly in microtiter plates. Assay conditions for each caspase have been optimized to obtain the maximal activity.