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Applications

  • Screening for apoptotic cells
  • Differentiating apoptotic and necrotic cells
  • Annexin detection
  • Flow cytometry
  • Fluorescence microscopy

Benefits

  • High fluorescence intensity and sensitivity
  • Many different labels and tags available
  • Appropriate for various multiwell formats and automation
  • Excitation and emission spectra are compatible with common excitation sources and filter sets

In the early stages of apoptosis, changes can be observed on the cell surface: Cells lose their phospholipid membrane asymmetry and expose phosphatidylserine (PS) on the exterior plasma membrane. Annexin V is a Ca2+-dependent, phospholipid-binding protein with high affinity for phosphatidylserine (PS) and is thus very useful for identifying apoptotic cells exposing PS on the cell surface to the extracellular environment. Annexin V may be conjugated to biotin or fluorophores such as FITC and phycoerythrin (PE) or it may be tagged with EGFP (Enhanced Green Fluorescent Protein). Since Annexin V conjugates or tagged Annexins retain their high affinity for PS, they are ideally suited as highly sensitive probes to identify early apoptotic cells and to detect apoptosis much earlier than assays based on DNA fragmentation or loss of membrane integrity. The stained cells can be evaluated by flow cytometry or fluorescence microscopy using common filter sets and flow cytometer channels.

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Annexin V Detection Kits

The Annexin V Apoptosis Detection Kits include an Annexin V conjugate or a tagged Annexin V, respectively, as well as binding buffer and partially also propidium iodide (PI) for differentiating between early apoptotic and necrotic cells. The kits allow a one-step staining procedure that takes only 10 minutes. In addition, the assay can be directly performed on living cells.

Promokine Annexin V Apoptosis Detection Kit plus includes an additional dye, SYTOX green, that is impermeant to live and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. After staining a cell population with e.g. fluorescent Annexin V-Cy3 or Annexin V-PE conjugates and SYTOX Green dye in the provided binding buffer, apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence. These populations can easily be distinguished by fluorescence microscopy using the appropriate filters.

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Individual Annexin V Reagents

PromoKine also offers a wide range of Annexin V reagents separately. For easy staining of early apoptotic cells, Annexin V conjugated to FITC, PE, Cy3, Cy5 and biotin are available, as well as Annexin V tagged with EGFP. The stained cells can be evaluated by flow cytometry or fluorescence microscopy using common filter sets and flow cytometer channels. Biotinylated Annexin V can be detected in conjunction with conventional dye-staining using any streptavidin- or avidin-dye reagents, such as our PromoFluor-streptavidin/avidin conjugates, fluorescein-, PE- or APC-streptavidin as well as streptavidin/avidin conjugated to peroxidase, alkaline phosphatase (AP), β-gal, etc.

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Order

Product Name Description Size Catalog Number Information Prices
Annexin V-FITC Detection Kit 100 assays PK-CA577-K101-100 click to select country
Annexin V-FITC Detection Kit 25 assays PK-CA577-K101-25 click to select country
Annexin V-FITC Detection Kit 400 assays PK-CA577-K101-400 click to select country
Annexin V-Cy3 Detection Kit 100 assays PK-CA577-K102-100 click to select country
Annexin V-Cy3 Detection Kit 25 assays PK-CA577-K102-25 click to select country
Annexin V-Cy3 Detection Kit 400 assays PK-CA577-K102-400 click to select country
Annexin V-Cy5 Detection Kit 100 assays PK-CA577-K103-100 click to select country
Annexin V-Cy5 Detection Kit 25 assays PK-CA577-K103-25 click to select country
Annexin V-Cy5 Detection Kit 400 assays PK-CA577-K103-400 click to select country
Annexin V-EGFP Detection Kit 100 assays PK-CA577-K104-100 click to select country
Annexin V-EGFP Detection Kit 25 assays PK-CA577-K104-25 click to select country
Annexin V-EGFP Detection Kit 400 assays PK-CA577-K104-400 click to select country
Annexin V-Biotin Detection Kit 100 assays PK-CA577-K109-100 click to select country
Annexin V-Biotin Detection Kit 25 assays PK-CA577-K109-25 click to select country
Annexin V-Biotin Detection Kit 400 assays PK-CA577-K109-400 click to select country
Annexin V-PE Detection Kit 100 assays PK-CA577-K128-100 click to select country
Annexin V-PE Detection Kit 25 assays PK-CA577-K128-25 click to select country
Annexin V-PE Detection Kit 400 assays PK-CA577-K128-400 click to select country
Annexin V-PE-Cy5 Detection Kit 100 assays PK-CA577-K129-100 click to select country
Annexin V-PE-Cy5 Detection Kit 25 assays PK-CA577-K129-25 click to select country
Annexin V-PE-Cy5 Detection Kit 400 assays PK-CA577-K129-400 click to select country
Annexin V-FITC Detection Kit Plus 100 assays PK-CA577-K201-100 click to select country
Annexin V-FITC Detection Kit Plus 25 assays PK-CA577-K201-25 click to select country
Annexin V-FITC Detection Kit Plus 400 assays PK-CA577-K201-400 click to select country
Annexin V-Cy3 Detection Kit pl Annexin V-Cy3 Detection Kit plus 100 assays PK-CA577-K202-100 click to select country
Annexin V-Cy3 Detection Kit pl Annexin V-Cy3 Detection Kit plus 25 assays PK-CA577-K202-25 click to select country
Annexin V-Cy3 Detection Kit pl Annexin V-Cy3 Detection Kit plus 400 assays PK-CA577-K202-400 click to select country
Annexin V-PE Detection Kit Plus 100 assays PK-CA577-K203-100 click to select country
Annexin V-PE Detection Kit Plus 25 assays PK-CA577-K203-25 click to select country
Annexin V-PE Detection Kit Plus 400 assays PK-CA577-K203-400 click to select country
Annexin V-FITC Reagent 1000 assays PK-CA577-1001-1000 click to select country
Annexin V-FITC Reagent 200 assays PK-CA577-1001-200 click to select country
Annexin V-Cy3 Reagent 1000 assays PK-CA577-1002-1000 click to select country
Annexin V-Cy3 Reagent 200 assays PK-CA577-1002-200 click to select country
Annexin V-Biotin Reagent 100 µg PK-CA577-1003-100 click to select country
Annexin V-Biotin Reagent 1 mg PK-CA577-1003-1000 click to select country
Annexin V-EGFP Reagent 1000 assays PK-CA577-1004-1000 click to select country
Annexin V-EGFP Reagent 200 assays PK-CA577-1004-200 click to select country
Annexin V Unlabeled Reagent 100 µg PK-CA577-1005-100 click to select country
10X Annexin V Binding Buffer 100 ml PK-CA577-1006 click to select country
Annexin V-Cy5 Reagent 1000 assays PK-CA577-1013-1000 click to select country
Annexin V-Cy5 Reagent 200 assays PK-CA577-1013-200 click to select country
Annexin V-PE Reagent 1000 assays PK-CA577-1014-1000 click to select country
Annexin V-PE Reagent 200 assays PK-CA577-1014-200 click to select country
Annexin V-PE-Cy5 Reagent 1000 assays PK-CA577-1015-1000 click to select country
Annexin V-PE-Cy5 Reagent 200 assays PK-CA577-1015-200 click to select country
1X Annexin V Binding Buffer 100 ml PK-CA577-1035 click to select country